immunocytochemical stain meaning in English
免疫细胞化学染色
Examples
- In the present experiment studies , an acute traumatic model of lateral cortical impact was employed to study expressive changes of microtubule associated protein - 2 ( map - 2 ) , cyclooxygenase - 2 ( cox - 2 ) , glial cell line - derived neurotrophic factor ( gdnf ) , caspase - 3 mrna and protein after brain injury in rats . immunocytochemical staining , western blotting , nucleic acid in situ hybridization with an oligonucleotide probe and computer image analysis were used to detect the dynamic changes of map - 2 mrna , cox - 2 mrna , gdnf mrna , and caspase - 3 mrna in the cortex after moderate traumatic brain injury ( tbi )
本实验从自行设计大鼠脑损伤落体打击器开始，先行建立了一个便于观察和施加处理因素、控制性好、重复性好的动物模型，选用30g击锤从25cm高处下落，冲击应力d为355 . 09kpa ，打击大鼠右顶部，造成中等程度的闭合性脑损伤，从病理形态学、组织超微结构观察及微管相关蛋白- 2 ( microtubuleassociatedprotein2 ， map - 2 ) 、环氧合酶- 2 ( cyclooxygenase2 ， cox - 2 ) 、胶质源性神经营养因子( glialcellline - derivedneutrophicfactor ， gdnf ) 、 caspase - 3基因及蛋白表达的时间性变化，详尽系统地阐述脑损伤后各指标变化的时间规律性及表达差异可能的形成机制。